특허 및 연구성과

Abstract

Stauntonia hexaphylla (Thunb.) Decne (SH), a medicinal plant from the Lardizabalaceae

family, holds traditional importance in East Asia for treating rheumatism. Steam treatment

is commonly applied to enhance its medicinal properties, but the chemical and biological

changes resulting from this process remain unexplored. This study compared steamed

and untreated SH fruit (SHF) extracts, analyzing their chemical composition, antioxidant

activity, and effects on bone health using in vitro models. Steamed SHF extracts exhibited

increased levels of 5-hydroxymethylfurfural (5-HMF), total flavonoids, phenolics, and

enhanced antioxidant activity. Bone health assessment using osteoclasts differentiated

from RAW 264.7 cells and osteoblasts from MC3T3-E1 cells revealed that steamed extracts

promoted alkaline phosphatase activity, calcium nodule formation, and collagen synthesis

in osteoblasts while inhibiting tartrate-resistant acid phosphatase (TRAP) activity in

osteoclasts. Additionally, steamed SHF extracts effectively modulated gene expression

related to osteoclastogenesis and osteoblastogenesis by downregulating TRAP, NFTAc1,

RANK, MMP9, c-Fos, and TRAF6 while upregulating ALP, Runx2, BGLAP, Col1a1, and

OPG. The component 5-HMF played a pivotal role in promoting alkaline phosphatase and

inhibiting TRAP activities. These findings suggest that steamed SHF may offer a promising

therapeutic approach for postmenopausal osteoporosis.